A simple assay for glutathione in whole blood.

Application of Riboflavin-Deficient Rat Erythrocytes in the in Vitro Assay of Faba Bean Toxicity

Background: Faba bean toxicity (favism) is a common condition observed in our region. Objective: To develop a short and simple technique involving the use of riboflavin-deficient rats to test in vivo faba bean toxicity. Methods/Results: Sprague Dawley rats were maintained on a riboflavin-deficient diet and their vitamin B2 status was monitored by the assay of erythrocyte glutathione reductase (...

Stereospecific Determination of Mefloquine in Whole Blood by HPLC

Mefloquine, as a racemic mixture, is used for the treatment and prophylaxis of malaria. Stereoselective pharmacodynamic and pharmacokinetic differences has been observed for mefloquine. In this study a modified stereoselective HPLC method is presented for determination of mefloquine (MFQ) enantiomers in whole blood. The assay involved liquid-liquid extraction of MFQ from biological fluids with ...

The Alterations of Plasma Iipid Peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase Enzyme Activities During Storage of Blood

Abstract Background and objective: Exposure of red blood cells to oxygen radicals can induce Lipid proxidation, hemoglobin damage and hemolysis of erythrocyte .The present study was designed to determine the alteration of plasma lipid peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities in stored blood and to find out the quantitative alterations and th...

A Rapid CE Assay for the Direct Quantification of Reduced and Oxidized Glutathione in Whole Blood

Evaluation of a modified salt-out method for DNA extraction from whole blood lymphocytes: A simple and economical method for gene polymorphism

Extraction of high-quality and-quantity DNA is a fundamental requirement for genetic research. It is very important to address the use of DNA extraction methods that are simple and cost-effective in gene polymorphism with large number of samples. This study was designed to investigate the optimal DNA extraction from lymphocytic cells by salt-out method. In this study, 200 blood samples of the t...